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	Provedor de dados BJID (1) BJM (1) Autor Gaspar,Ana Maria Coimbra (2) Miagostovich,Marize Pereira (1) Paula,Vanessa Salete de (1) Prado,Tatiana (1) Villar,Livia Melo (1) Palavra-chave Activated sludge process (1) Enteric viruses (1) Extraction methods (1) HAV (1) RT-PCR (1) Serum and stool (1) Sewage sludge (1) Virus concentration methods (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2014 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Comparison of four extraction methods to detect hepatitis A virus RNA in serum and stool samples BJID Paula,Vanessa Salete de; Villar,Livia Melo; Gaspar,Ana Maria Coimbra. The efficiency of extraction methods for hepatitis A virus (HAV) RNA in clinical samples is of great importance for molecular diagnosis, especially in regions endemic for HAV, such as Brazil. We compared the efficiency of four different extraction techniques in serum and stool samples for the detection of hepatitis A virus by reverse transcription PCR (RT-PCR). We used PCR to analyse serum and stool samples of 12 patients who were referred to the Brazilian Reference Center for Viral Hepatitis (BRCVH) in Rio de Janeiro. The methods tested were Proteinase K, Silica, TRIzol and Guanidine isothiocyanate. Proteinase K extraction was the best method for serum samples; it detected the HAV-RNA in 11 of the 12 samples. The guanidine isothiocyanate method was the... Tipo: Info:eu-repo/semantics/article Palavras-chave: HAV; Extraction methods; Serum and stool; RT-PCR. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000200007 Detection of enteric viruses in activated sludge by feasible concentration methods BJM Prado,Tatiana; Gaspar,Ana Maria Coimbra; Miagostovich,Marize Pereira. Human enteric viruses are responsible to cause several diseases, including gastroenteritis and hepatitis, and can be present in high amounts in sewage sludge. This study compared virus recovery efficiency of two feasible concentration methods used for detecting human adenovirus (HAdV), rotavirus species A (RV-A), norovirus genogroup II (NoV GII) and hepatitis A virus (HAV) in sewage sludge from an activated sludge process. Twelve sewage sludge samples were collected bi-monthly from January to July, 2011. Ultracentrifugation was compared with a simplified protocol based on beef extract elution for recovering enteric viruses. Viruses were quantified by quantitative real-time PCR assays and virus recovery efficiency and limits of detection were determined.... Tipo: Info:eu-repo/semantics/other Palavras-chave: Enteric viruses; Sewage sludge; Activated sludge process; Virus concentration methods. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100049 Registros recuperados: 2 Primeira ... 1 ... Última

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